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KMID : 0606320040210010104
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Journal of Phamacetical Sciences Sookmyung Women s University
2004 Volume.21 No. 1 p.104 ~ p.108
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Effects of Prostaglandin E2 on osteoclast formation
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Cho Eun-Sook
Yim Mi-Jung
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Abstract
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Osteoclasts, the multinucleated giant cells that resort bone, develop from hemopoietic cells of monocyte-macrophage lineage. Differentiation of osteoclasts is strictly regulated by osteoblasts, which deposit bone. In the present study, prostaglandin E2 (PGE2) induced osteoclast formation in co-cultures of mouse bone marrow cells and calvarial osteoblasts. This activity was not observed in the absence of calvarial osteoblasts, suggesting that calvarial osteoblasts are likely target cells of PGE2. Northern blot analysis revealed that the effect of PGE2 is associated with increased expression of the osteoclast differentiation factor TNF-related activation-induced cytokine (TRANCE, identical to RANKL, ODF, and OPGL) in mouse calvarial osteoblasts. Induction of TRANCE expression by PGE2 was partially suppressed by the inhibitors of ERK and p38 MAPK, suggesting that activation of ERK and p38 MAPK is involved in TRANCE expression by PGE2.
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